一种针对外周苯二氮䓬受体的单克隆抗体可激活人中性粒细胞NADPH氧化酶。

A monoclonal antibody against peripheral benzodiazepine receptor activities the human neutrophil NADPH-oxidase.

作者信息

Zavala F, Masson A, Brys L, de Baetselier P, Descamps-Latscha B

机构信息

INSERM U25, Hôpital Necker, Paris, France.

出版信息

Biochem Biophys Res Commun. 1991 May 15;176(3):1577-83. doi: 10.1016/0006-291x(91)90468-m.

DOI:10.1016/0006-291x(91)90468-m

PMID:1645547

Abstract

A murine monoclonal antibody, mAb 8523, raised against whole human pro-monocytic U937 cells recognizes an 18 kDa antigen in human neutrophils (PMN), as determined by immunoprecipitation and by immunodetection on Western blots of SDS-PAGE of PMN membrane fractions. That is 18 kDa antigen corresponds to the phagocyte peripheral benzodiazepine receptor (PBZDR) is evidenced by its co-migration with the 18 kDa covalently labeled PBZDR, detected by autoradiography, and their co-modulation upon phorbol-myristate-acetate activation of PMN. Purified mAb 8523 (IgG2b) is able to dose-dependently and specifically stimulate both the basal and the FMLP-induced oxidative burst of intact human PMN, assessed by luminol-amplified chemiluminescence. This property of the first described monoclonal antibody against PBZDR supports the implication of this receptor in NADPH-oxidase activation and consequently in phagocyte-dependent host defense mechanisms.

摘要

一种针对整个人类原单核细胞U937细胞产生的鼠单克隆抗体mAb 8523，通过免疫沉淀和对中性粒细胞（PMN）膜组分的SDS-PAGE进行Western印迹免疫检测，可识别PMN中的一种18 kDa抗原。该18 kDa抗原与吞噬细胞外周苯二氮䓬受体（PBZDR）相对应，这一点可通过其与经放射自显影检测的18 kDa共价标记PBZDR的共迁移以及在PMN经佛波酯-肉豆蔻酸酯-乙酸酯激活后的共调节来证明。纯化的mAb 8523（IgG2b）能够剂量依赖性且特异性地刺激完整人类PMN的基础和FMLP诱导的氧化爆发，通过鲁米诺增强化学发光进行评估。这种针对PBZDR的首个描述的单克隆抗体的特性支持了该受体在NADPH氧化酶激活以及因此在吞噬细胞依赖性宿主防御机制中的作用。