Wirthmueller U, Baggiolini M, de Weck A L, Dahinden C A
Institute of Clinical Immunology, University of Bern, Switzerland.
Biochem Biophys Res Commun. 1991 May 15;176(3):972-8. doi: 10.1016/0006-291x(91)90377-j.
We examined the production of PAF and LTB4 by PMN in response to NAP1/IL-8 alone, or after preincubation with GM-CSF, which has been shown to enhance PMN responsiveness and to prime PMN for production of those bioactive lipids. NAP-1/IL-8 does not induce the synthesis of PAF and LTB4 from endogenous phospholipid precursors, even after preincubation with GM-CSF. In addition and again in contrast to fMLP and C5a, NAP-1/IL-8 fails to induce an enhanced oxidative burst in GM-CSF primed PMN. Exogenously added PAF or LTB4 can mimic the priming effect of GM-CSF for an enhanced oxidative burst in response to all examined chemotactic peptides including NAP1/IL-8. Our data reveal a possible autocrine role of PAF and LTB4 in the enhanced responsiveness of GM-CSF primed PMN towards fMLP or C5a, but not NAP-1/IL-8.
我们检测了中性粒细胞（PMN）对单独的NAP1/IL-8或与GM-CSF预孵育后产生PAF和LTB4的情况，GM-CSF已被证明可增强PMN的反应性并使PMN为产生这些生物活性脂质做好准备。即使与GM-CSF预孵育后，NAP-1/IL-8也不会从内源性磷脂前体诱导PAF和LTB4的合成。此外，与fMLP和C5a再次形成对比的是，NAP-1/IL-8未能在GM-CSF预处理的PMN中诱导增强的氧化爆发。外源性添加的PAF或LTB4可模拟GM-CSF对包括NAP1/IL-8在内的所有检测趋化肽增强氧化爆发的预处理作用。我们的数据揭示了PAF和LTB4在GM-CSF预处理的PMN对fMLP或C5a而非NAP-1/IL-8增强反应性中可能的自分泌作用。
