Doyle L A, Mabry M, Stahel R A, Waibel R, Goldstein L H
University of Maryland Cancer Centre, Baltimore.
Br J Cancer Suppl. 1991 Jun;14:39-42.
Small cell lung cancer (SCLC) manifests a number of neuroendocrine differentiation features and antigenic characteristics that distinguish the tumour from non-small cell lung cancer (NSCLC). Several surface antigens on SCLC cells, identified by clusters of monoclonal antibodies (MAbs), distinguish SCLC and other neuroendocrine tumours of NSCLC. Stable transfection of the c-myc proto-oncogene has been reported to confer upon classic SCLC cells the growth properties and morphology of the variant subtype of SCLC (SCLC-v). Furthermore, insertion of the v-Ha-ras oncogene into such SCLC-v cells has been found to induce features typical of NSCLC. We have used classic SCLC cells transfected with c-myc, or co-transformed with c-myc and v-Ha-ras, to examine the expression of characteristics SCLC cluster antigens. Flow cytometric assays reveal that SCLC cells co-transformed with c-myc and v-Ha-ras oncogenes down-modulate SC-1, SC-2 and SC-5A surface antigens to levels approaching, in some cases, those seen with NSCLC cells. The SC-4 surface antigen is not modulated by activation of these oncogenes. These findings support clinical and laboratory observations that important transitions can occur between subtypes of human lung cancer cells, and that these shifts may play a role in the clinical progression of lung cancer.
小细胞肺癌（SCLC）表现出许多神经内分泌分化特征和抗原特性，这些特征将该肿瘤与非小细胞肺癌（NSCLC）区分开来。通过单克隆抗体（MAb）簇鉴定的SCLC细胞上的几种表面抗原，可区分SCLC和NSCLC的其他神经内分泌肿瘤。据报道，c-myc原癌基因的稳定转染赋予经典SCLC细胞SCLC变异亚型（SCLC-v）的生长特性和形态。此外，已发现将v-Ha-ras癌基因插入此类SCLC-v细胞可诱导NSCLC的典型特征。我们使用转染了c-myc的经典SCLC细胞，或与c-myc和v-Ha-ras共转化的细胞，来检测特征性SCLC簇抗原的表达。流式细胞术分析显示，与c-myc和v-Ha-ras癌基因共转化的SCLC细胞下调SC-1、SC-2和SC-5A表面抗原的水平，在某些情况下接近NSCLC细胞的水平。SC-4表面抗原不受这些癌基因激活的调节。这些发现支持了临床和实验室观察结果，即人类肺癌细胞亚型之间可能发生重要转变，并且这些转变可能在肺癌的临床进展中起作用。
