Numao T, Fukuda T, Akutsu I, Makino S
Department of Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1991 Jan;29(1):65-71.
To evaluate the acute effects of anti-asthmatic drugs in vitro, we examined the modulation of various anti-asthmatic drugs in therapeutic concentrations on PAF-induced human eosinophil chemotaxis. Aminophylline (20 micrograms/ml) and Isoproterenol (10 nM) inhibited PAF (3 X 10(-8) M)-induced eosinophil chemotaxis nearly 30%, whereas no inhibitory effects were observed by Dexamethasone (0.1 microM), Tranilast, Ketotifen or Azelastine. Aminophylline (20 micrograms/ml) also inhibited LTB4 (3 X 10(-8) M)-induced eosinophil chemotaxis nearly 30%, whereas it did not inhibit chemotaxis induced by zymosan (5 mg/ml)-activated serum. These results indicate that anti-asthmatic drugs except for aminophylline and isoproterenol, when used acutely in therapeutic concentrations, have no striking inhibitory effects on PAF-induced eosinophil chemotaxis. These results further suggest the possibility that there are different mechanisms in eosinophil chemotaxis induced by PAF, LTB4 or by C5a.
为了评估抗哮喘药物的急性体外效应，我们检测了治疗浓度下各种抗哮喘药物对血小板活化因子（PAF）诱导的人嗜酸性粒细胞趋化性的调节作用。氨茶碱（20微克/毫升）和异丙肾上腺素（10纳摩尔）抑制PAF（3×10⁻⁸摩尔）诱导的嗜酸性粒细胞趋化性近30%，而地塞米松（0.1微摩尔）、曲尼司特、酮替芬或氮卓斯汀未观察到抑制作用。氨茶碱（20微克/毫升）也抑制白三烯B4（LTB4，3×10⁻⁸摩尔）诱导的嗜酸性粒细胞趋化性近30%，但不抑制酵母聚糖（5毫克/毫升）激活血清诱导的趋化性。这些结果表明，除氨茶碱和异丙肾上腺素外，抗哮喘药物在治疗浓度下急性使用时，对PAF诱导的嗜酸性粒细胞趋化性没有显著抑制作用。这些结果进一步提示，PAF、LTB4或C5a诱导嗜酸性粒细胞趋化性可能存在不同机制。
