Wang Haiyan, Ye Qingfu, Wang Wei, Wu Licheng, Wu Weixiang
Institute of Nuclear Agricultural Sciences, Zhejiang University, Hua-jia-chi Campus, Hangzhou 310029, China.
Environ Pollut. 2006 Oct;143(3):449-55. doi: 10.1016/j.envpol.2005.12.006. Epub 2006 Feb 3.
Expression of Cry1Ab protein in Bt transgenic rice (KMD) and its residue in the rhizosphere soil during the whole growth in field, as well as degradation of the protein from KMD straw in five soils under laboratory incubation were studied. The residue of Cry1Ab protein in KMD rhizosphere soil was undetectable (below the limit of 0.5 ng/g air-dried soil). The Cry1Ab protein contents in the shoot and root of KMD were 3.23-8.22 and 0.68-0.89 microg/g (fresh weight), respectively. The half-lives of the Cry1Ab protein in the soils amended with KMD straw (4%, w/w) ranged from 11.5 to 34.3d. The residence time of the protein varied significantly in a Fluvio-marine yellow loamy soil amended with KMD straw at the rate of 3, 4 and 7%, with half-lives of 9.9, 13.8 and 18d, respectively. In addition, an extraction method for Cry1Ab protein in soil was developed, with extraction efficiencies of 46.4-82.3%.
研究了转Bt基因水稻（KMD）中Cry1Ab蛋白在田间全生育期的表达及其根际土壤中的残留情况，以及实验室培养条件下KMD稻草中的该蛋白在五种土壤中的降解情况。KMD根际土壤中Cry1Ab蛋白残留未检测到（低于0.5 ng/g风干土的检测限）。KMD地上部和根部的Cry1Ab蛋白含量分别为3.23 - 8.22和0.68 - 0.89 μg/g（鲜重）。添加KMD稻草（4%，w/w）的土壤中Cry1Ab蛋白的半衰期为11.5至34.3天。在添加3%、4%和7% KMD稻草的滨海潮土中，该蛋白的停留时间差异显著，半衰期分别为9.9、13.8和18天。此外，还建立了一种土壤中Cry1Ab蛋白的提取方法，提取效率为46.4 - 82.3%。
