急性可卡因对大鼠纹状体中多巴胺转运体调节相关基因的调控
Regulation of genes involved in dopamine transporter modulation by acute cocaine in rat striatum.
作者信息
Courtin Cindie, Crete Dominique, Canestrelli Corinne, Noble Florence, Marie-Claire Cynthia
机构信息
CNRS UMR 7157, Paris F-75006, France.
出版信息
Neurosci Lett. 2006 May 8;398(3):235-40. doi: 10.1016/j.neulet.2006.01.001. Epub 2006 Feb 3.
Abstract
It is well established that acute administration of psychostimulants alters dopamine transport. However, the exact mechanism of this modulation is still unknown. In this study we examined the mRNA levels of several proteins involved in the various proposed processes following cocaine administration. The expression levels of several immediate early genes were also studied. This was performed in rat striatum using real-time quantitative PCR. As expected, a marked increase of the immediate early genes Fos, Egr1 and Egr3 was observed. Egr2 was also found up-regulated. Among the different genes studied only Synaptotagmin4 in the SNARE family and Synphilin1 in the synaptic vesicles binding family were modulated by acute cocaine treatment. Interestingly, acute amphetamine treatment did not increase either Synaptotagmin4 and Synphilin1 mRNA levels, although increases in early genes expression were noted.
摘要
众所周知，急性给予精神兴奋剂会改变多巴胺转运。然而，这种调节的确切机制仍然未知。在本研究中，我们检测了可卡因给药后参与各种提出的过程的几种蛋白质的mRNA水平。还研究了几种即刻早期基因的表达水平。这是在大鼠纹状体中使用实时定量PCR进行的。正如预期的那样，观察到即刻早期基因Fos、Egr1和Egr3有显著增加。还发现Egr2上调。在所研究的不同基因中，只有SNARE家族中的突触结合蛋白4和突触小泡结合家族中的突触素1受到急性可卡因治疗的调节。有趣的是，急性苯丙胺治疗虽然注意到早期基因表达增加，但并未增加突触结合蛋白4和突触素1的mRNA水平。
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