仅下丘脑室旁核中的精氨酸加压素而非催产素和内源性阿片肽在大鼠针刺镇痛中发挥作用。
Only arginine vasopressin, not oxytocin and endogenous opiate peptides, in hypothalamic paraventricular nucleus play a role in acupuncture analgesia in the rat.
作者信息
Yang Jun, Liu Wen-yan, Song Cao-you, Lin Bao-cheng
机构信息
Department of Nuclear Medicine, Nanfang Hospital, Guangzhou 510515, China.
出版信息
Brain Res Bull. 2006 Feb 15;68(6):453-8. doi: 10.1016/j.brainresbull.2005.10.002. Epub 2005 Oct 21.
Our previous study proved that hypothalamic paraventricular nucleus (PVH) plays an important role in acupuncture analgesia. The effect of acupuncture on the concentrations of arginine vasopressin (AVP), oxytocin (OXT), leucine-enkephaline (L-Ek), beta-endorphin (beta-Ep) and dynorphinA(1-13) (DynA(1-13)) was investigated in rat PVH. Electrical acupuncture of "Zusanli" points (St. 36) 30 min increased the AVP, not OXT, L-Ek, beta-Ep and DynA(1-13) concentrations in PVH tissue using micropunch and radioimmunoassay, which showed a negative relationship between the pain threshold and AVP concentrations in PVH tissue. Electrical acupuncture could elevate the AVP concentrations in PVH perfuse liquid during acupuncture, and then reduce the AVP concentrations in PVH perfuse liquid after acupuncture. But no change in OXT, L-Ek, beta-Ep and DynA(1-13) concentrations was detected in PVH perfuse liquid. Electrical acupuncture decreased the number of AVP, not OXT, L-Ek, beta-Ep and DynA(1-13) immunoreactive cells in PVH using immunocytochemistry. The results suggested that only AVP, not OXT and endogenous opiate peptides in PVH involved acupuncture analgesia in the rat.
我们之前的研究证明，下丘脑室旁核（PVH）在针刺镇痛中起重要作用。本研究观察针刺对大鼠PVH中精氨酸加压素（AVP）、催产素（OXT）、亮氨酸脑啡肽（L-Ek）、β-内啡肽（β-Ep）和强啡肽A（1-13）（DynA（1-13））含量的影响。采用微量打孔和放射免疫分析法，电针“足三里”穴30分钟可使PVH组织中AVP含量升高，而OXT、L-Ek、β-Ep和DynA（1-13）含量无变化，且PVH组织中痛阈与AVP含量呈负相关。电针过程中可使PVH灌流液中AVP含量升高，电针后则降低，但PVH灌流液中OXT、L-Ek、β-Ep和DynA（1-13）含量无变化。免疫细胞化学法显示，电针可使PVH中AVP免疫反应阳性细胞数减少，而OXT、L-Ek、β-Ep和DynA（1-13）免疫反应阳性细胞数无变化。结果提示，在大鼠针刺镇痛过程中，仅PVH中的AVP参与，而OXT和内源性阿片肽不参与。
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