Laugier C, Zebda N, Fanidi A, Lagarde M, Pageaux F
INSERM U.205, INSA, Villeurbanne, France.
Biochem Biophys Res Commun. 1991 May 31;177(1):324-9. doi: 10.1016/0006-291x(91)91986-m.
Regulation of calmodulin-independent and -dependent cAMP phosphodiesterases from quail oviduct by various fatty acids was studied. The calmodulin-independent form was slightly activated by low concentrations (20 microM) of oleic, linoleic and arachidonic acid, higher concentrations were inhibitory. The basal activity of the calmodulin-dependent form was activated by linoleic acid and to a lesser extent by arachidonic acid at low concentrations and inhibited by higher concentrations of the two fatty acids. In contrast, arachidonic acid was a potent reversible inhibitor of calmodulin in the activation of this enzyme (IC50: 20 microM) whereas linoleic acid was inactive from 10 to 150 microM. The present results strongly suggest that the differential regulation of cAMP phosphodiesterases by these fatty acids could profoundly influence the level of cAMP in the oviduct and thus its subsequent effects.
研究了鹌鹑输卵管中钙调蛋白非依赖性和依赖性环磷酸腺苷磷酸二酯酶受各种脂肪酸的调控情况。低浓度（20微摩尔）的油酸、亚油酸和花生四烯酸对钙调蛋白非依赖性形式有轻微激活作用，高浓度则有抑制作用。钙调蛋白依赖性形式的基础活性在低浓度时被亚油酸激活，花生四烯酸在较小程度上也有激活作用，而高浓度的这两种脂肪酸则有抑制作用。相反，花生四烯酸是钙调蛋白激活该酶时的一种强效可逆抑制剂（IC50：20微摩尔），而亚油酸在10至150微摩尔时无活性。目前的结果强烈表明，这些脂肪酸对环磷酸腺苷磷酸二酯酶的差异调控可能会深刻影响输卵管中环磷酸腺苷的水平，进而影响其后续效应。
