Dootz Rolf, Nie Jingjing, Du Binyang, Herminghaus Stephan, Pfohl Thomas
Applied Physics Department, University of Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany.
Langmuir. 2006 Feb 14;22(4):1735-41. doi: 10.1021/la052739y.
We analyze microstructured multilayer films of poly(ethyleneimine) (PEI) and DNA by employing Raman and surface enhanced Raman spectroscopy (SERS). The microstructuring of the samples allows a simultaneous measurement of signal and reference in a single analytic process. Silver nanoparticles are implemented in the microstructured multilayers for SERS measurements. The recorded SERS spectra of PEI/DNA are dominated by the Raman bands of the DNA bases which show a larger mean enhancement than bands belonging to DNA backbone vibrations. Our results show that the combination of SERS and microstructured multilayer films provides an adapted way to characterize the polyelectrolytes as well as to measure the enhancement factor and the distance dependence for the SERS active silver nanoparticles. Furthermore, microstructured polyelectrolyte films containing SERS active nanoparticles are used for sensing molecules.
我们通过拉曼光谱和表面增强拉曼光谱（SERS）分析了聚（乙烯亚胺）（PEI）和DNA的微结构多层膜。样品的微结构化使得在单个分析过程中能够同时测量信号和参考信号。在微结构多层膜中引入银纳米颗粒用于SERS测量。记录的PEI/DNA的SERS光谱主要由DNA碱基的拉曼带主导，这些拉曼带显示出比属于DNA主链振动的带更大的平均增强。我们的结果表明，SERS和微结构多层膜的结合提供了一种适用于表征聚电解质以及测量SERS活性银纳米颗粒的增强因子和距离依赖性的方法。此外，含有SERS活性纳米颗粒的微结构聚电解质膜用于传感分子。
