Wang Zhuyuan, Bonoiu Adela, Samoc Marek, Cui Yiping, Prasad Paras N
Institute for Lasers, Photonics and Biophotonics, SUNY at Buffalo, Buffalo, NY 14260, United States.
Biosens Bioelectron. 2008 Jan 18;23(6):886-91. doi: 10.1016/j.bios.2007.09.017. Epub 2007 Sep 29.
We report pH sensing for biological applications based on surface enhanced Raman scattering (SERS) from silver nanoparticles functionalized with 2-aminothiophenol (2-aminobenzenethiol, 2-ABT). pH-dependent SERS spectra of the attached 2-ABT molecules enable one to sense the pH over the range of 3.0-8.0. We have performed the first demonstration of SERS detection in living cells kept in different pH buffer solutions and show that the pH sensitivity is retained in that case. Thus, the nanoparticles can be used as probes delivering spatially localized chemical information from biological environments and provide a new way to monitor chemical changes at cellular level.
我们报道了基于用2-氨基硫酚（2-氨基苯硫酚，2-ABT）功能化的银纳米颗粒的表面增强拉曼散射（SERS）用于生物应用的pH传感。附着的2-ABT分子的pH依赖性SERS光谱能够使人在3.0 - 8.0的范围内检测pH值。我们首次在置于不同pH缓冲溶液中的活细胞中进行了SERS检测演示，并表明在这种情况下pH敏感性得以保留。因此，这些纳米颗粒可用作从生物环境中传递空间局部化学信息的探针，并提供一种在细胞水平监测化学变化的新方法。
