Sarzani R, Arnaldi G, Chobanian A V
Whitaker Cardiovascular Institute, Boston, Mass.
Hypertension. 1991 Jun;17(6 Pt 2):888-95. doi: 10.1161/01.hyp.17.6.888.
Hypertension-associated growth of vascular smooth muscle cells might be mediated in vivo by platelet-derived growth factor (PDGF). Our previous investigations in hypertensive rats failed to demonstrate changes in aortic steady-state mRNA levels of PDGF A or B chains. The current studies were performed to determine whether hypertension might affect the expression of PDGF receptors. We studied PDGF alpha- and beta-receptor gene expression by Northern analysis using human and rat cDNA probes. Studies of tissue distribution revealed that PDGF beta-receptor mRNA was most abundant in total aorta and aortic media, whereas the PDGF alpha-receptor mRNA was most abundant in the lung and was expressed at low levels in aortic tissue. Deoxycorticosterone acetate (DOCA)-salt hypertension induced a threefold increase in aortic steady-state PDGF beta-receptor mRNA levels. Aortic PDGF beta-receptor expression also was higher in spontaneously hypertensive rats (SHRs) when compared with age-matched normotensive Wistar-Kyoto (WKY) controls. Aortic PDGF alpha-receptor steady-state mRNA levels were unchanged in DOCA-salt hypertension and were expressed at similar levels in WKY rats and SHRs. Unlike the findings with aorta, cardiac PDGF beta- and alpha-receptor and PDGF B-chain expressions were unchanged in the DOCA-salt model and were decreased in SHRs. These findings indicate that hypertension can increase aortic steady-state mRNA levels for PDGF beta-receptor. They also indicate that tissue-specific expression of the genes of the PDGF ligand/receptor system are differentially regulated in hypertension.
高血压相关的血管平滑肌细胞生长可能在体内由血小板衍生生长因子（PDGF）介导。我们之前对高血压大鼠的研究未能证明主动脉中PDGF A或B链的稳态mRNA水平有变化。进行当前研究以确定高血压是否可能影响PDGF受体的表达。我们使用人和大鼠cDNA探针通过Northern分析研究了PDGFα和β受体基因的表达。组织分布研究表明，PDGFβ受体mRNA在主动脉全层和主动脉中层中最为丰富，而PDGFα受体mRNA在肺中最为丰富，在主动脉组织中表达水平较低。醋酸脱氧皮质酮（DOCA）-盐性高血压使主动脉稳态PDGFβ受体mRNA水平增加了三倍。与年龄匹配的正常血压Wistar-Kyoto（WKY）对照相比，自发性高血压大鼠（SHR）的主动脉PDGFβ受体表达也更高。在DOCA-盐性高血压中，主动脉PDGFα受体稳态mRNA水平未发生变化，并且在WKY大鼠和SHR中表达水平相似。与主动脉的研究结果不同，在DOCA-盐模型中，心脏PDGFβ和α受体以及PDGF B链的表达未发生变化，而在SHR中则降低。这些发现表明高血压可增加主动脉中PDGFβ受体的稳态mRNA水平。它们还表明，在高血压中，PDGF配体/受体系统基因的组织特异性表达受到不同的调节。
