Ranjard L, Lignier L, Chaussod R
INRA/Université de Bourgogne, UMR Microbiologie et Géochimie des Sols, CMSE, 17 rue de Sully, B.V. 86510, 21065 Dijon Cedex, France.
Appl Environ Microbiol. 2006 Feb;72(2):1684-7. doi: 10.1128/AEM.72.2.1684-1687.2006.
In this study we evaluated the short-term effects of copper, cadmium, and mercury, added singly or in combination at different doses, on soil bacterial community structure using the bacterial automated ribosomal intergenic spacer analysis (B-ARISA) fingerprinting technique. Principal-component analysis of B-ARISA profiles allowed us to deduce the following order of impact: (Cu + Cd + Hg) >> Hg > or = Cd > Cu. These results demonstrated that there was a cumulative effect of metal toxicity. Furthermore, the trend of modifications was consistent with the "hump-backed" relationships between biological diversity and disturbance described by Giller et al. (K. E. Giller, E. Witler, and S. P. McGrath, Soil Biol. Biochem. 30:1389-1414, 1998).
在本研究中,我们使用细菌自动核糖体基因间隔区分析(B-ARISA)指纹技术,评估了不同剂量的铜、镉和汞单独或组合添加对土壤细菌群落结构的短期影响。对B-ARISA图谱进行主成分分析,使我们能够推断出以下影响顺序:(铜+镉+汞)>>汞≥镉>铜。这些结果表明存在金属毒性的累积效应。此外,变化趋势与吉勒等人描述的生物多样性与干扰之间的“驼峰状”关系一致(K.E.吉勒、E.维特勒和S.P.麦格拉思,《土壤生物学与生物化学》30:1389 - 1414,1998年)。