环境应用中功能基因微阵列寡核苷酸探针设计标准的改进。
Improvement of oligonucleotide probe design criteria for functional gene microarrays in environmental applications.
作者信息
Liebich Jost, Schadt Christopher W, Chong Song C, He Zhili, Rhee Sung-Keun, Zhou Jizhong
机构信息
Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
出版信息
Appl Environ Microbiol. 2006 Feb;72(2):1688-91. doi: 10.1128/AEM.72.2.1688-1691.2006.
Abstract
To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (< or =90%), the length of identical sequence stretches (< or =20 bases), and the binding free energy (> or =-35 kcal mol(-1)) was found to be predictive of probe specificity.
摘要
为优化寡核苷酸探针设计标准，将与探针具有不同相似性的PCR产物与用于检测不同生物体同源基因的功能基因微阵列进行杂交。与基于单一标准的更严格探针设计不同，同时考虑相似百分比（≤90%）、相同序列延伸长度（≤20个碱基）和结合自由能（≥ -35 kcal mol⁻¹）被发现可预测探针特异性。
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