Markauskas A, Tiknius V, Marcisauskas R
Institute of Applied Enzymology FERMENTAS, Lithuania, U.S.S.R.
J Chromatogr. 1991 Feb 22;539(2):525-9. doi: 10.1016/s0021-9673(01)83964-9.
The interaction between TdT and Topo I, and twelve various triazine dyes immobilized on Sepharose CL-6B was studied. Yellow lightproof 2KT-Sepharose and Bordeaux 4ST-Sepharose were used to purify TdT and Topo I, respectively. The principal role of copper ions, complexed to the dye molecules, in the dye-protein interaction was evaluated.
研究了末端脱氧核苷酸转移酶（TdT）与拓扑异构酶I（Topo I）以及固定在琼脂糖CL-6B上的十二种不同三嗪染料之间的相互作用。分别使用耐黄2KT-琼脂糖和波尔多4ST-琼脂糖来纯化TdT和Topo I。评估了与染料分子络合的铜离子在染料-蛋白质相互作用中的主要作用。
