Pfleiderer M, Routledge E, Herrler G, Siddell S G
Institute of Virology, University of Würzburg, Germany.
J Gen Virol. 1991 Jun;72 ( Pt 6):1309-15. doi: 10.1099/0022-1317-72-6-1309.
We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemadsorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.
我们已在痘苗病毒/T7 RNA 聚合酶系统中表达了鼠冠状病毒血凝素酯酶蛋白。观察到的表达水平显著高于在病毒感染细胞中发现的水平。所表达的蛋白具有破坏受体（酯酶）和结合受体（血细胞吸附）的活性。该系统的使用将极大地促进对该蛋白结构-功能关系的分析。
