Akashi Satoko
International Graduate School of Arts and Sciences, Yokohama City University, Tsurumi-ku, Kanagawa, Japan.
Med Res Rev. 2006 May;26(3):339-68. doi: 10.1002/med.20051.
The advent of electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) has accelerated structural studies of biological macromolecular complexes. At present, mass spectrometry can provide accurate mass values not only of individual biological macromolecules but also of their assemblies. Furthermore, it can also give information on the interface sites of the biological macromolecular complexes. The present article focuses on the role of mass spectrometry in the investigation of biological molecular interactions, such as protein-protein, protein-DNA, and protein-ligand interactions, which play essential roles in various biological events.
电喷雾电离（ESI）和基质辅助激光解吸电离（MALDI）的出现加速了对生物大分子复合物的结构研究。目前，质谱不仅可以提供单个生物大分子的精确质量值，还能提供其组装体的精确质量值。此外，它还能给出有关生物大分子复合物界面位点的信息。本文重点关注质谱在研究生物分子相互作用中的作用，如蛋白质-蛋白质、蛋白质-DNA和蛋白质-配体相互作用，这些相互作用在各种生物事件中起着至关重要的作用。
