Gong Nianqiao, Li Guoxun, Xiao Jiansheng, Guo Hui, Ye Qifa
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2005;25(5):555-7. doi: 10.1007/BF02896016.
The change and the role of MAPK cascade pathway and P53 pathway after liver transplantation were explored. Thirty-four punctured donor liver specimens and 10 normal liver specimens were classified as group A (no rejection, n = 10), group B (mild/moderate acute rejection, n = 10), group C (serious acute rejection, n = 8), group D (chronic rejection/fibrosis, n = 6) and group E (control, n = 10). By using immunohistochemistry, the expression levels of mitogen activated protein kinase (MAPK), Ras and P53 proteins, and by in situ hybridization, MAPK and ras mRNA expression levels were detected. The results showed that the expression levels of MAPK and Ras proteins were increased by turns in groups A, B and C, and decreased by turns in groups D and E. The protein expression of P53 was higher in the treated groups. The expression of Ras, HSP70 mRNA was identical as that of protein. It is suggested that the MAPK cascade pathway and P53 pathway can protect the hepatocytes by different mechanisms after liver transplantation. MAPKs cascade pathway repairs hepatocyte injury or accelerates hepatocytes into proliferation or differentiation. P53 pathway blocks cell cycle within G1 phase to make hepatocyte repair or apoptosis to reduce disorder differentiation.
探讨肝移植后丝裂原活化蛋白激酶（MAPK）级联通路和P53通路的变化及作用。将34例穿刺获取的供肝标本和10例正常肝标本分为A组（无排斥反应，n = 10）、B组（轻度/中度急性排斥反应，n = 10）、C组（重度急性排斥反应，n = 8）、D组（慢性排斥反应/纤维化，n = 6）和E组（对照组，n = 10）。采用免疫组织化学法检测丝裂原活化蛋白激酶（MAPK）、Ras和P53蛋白的表达水平，采用原位杂交法检测MAPK和ras mRNA表达水平。结果显示，MAPK和Ras蛋白表达水平在A、B、C组依次升高，在D、E组依次降低。P53蛋白在各处理组表达较高。Ras、HSP70 mRNA的表达与蛋白表达一致。提示肝移植后MAPK级联通路和P53通路可通过不同机制保护肝细胞。MAPK级联通路修复肝细胞损伤或加速肝细胞增殖或分化。P53通路在G1期阻断细胞周期，使肝细胞修复或凋亡以减少分化紊乱。
