Dobrucki J W, Sutherland R M, Swartz H M
University of Illinois, Illinois Electron Paramagnetic Resonance Research Center, Urbana 61801.
Magn Reson Med. 1991 May;19(1):42-55. doi: 10.1002/mrm.1910190105.
Electron paramagnetic resonance imaging (EPRI) and EPR spectroscopy of nitroxides have been applied to detect and quantify cytotoxic effects in mammalian cells in vitro. Images depicting microscopic viable and nonviable areas in the same multicellular spheroid (a model of solid tumors) have been acquired prior to and following the treatment with an antitumor drug, Adriamycin. The loss of viability in the inner region of the viable rim was detected. Such mapping of the time-course changes of the localization of viable and nonviable cells in the same intact biological object is not possible with routinely used methods to measure viability, such as histological examination. The experimental conditions required for high accuracy and sensitivity of the EPRI of spheroids have been evaluated and directions for further development of this approach are indicated.
电子顺磁共振成像（EPRI）和氮氧化物的电子顺磁共振光谱已被用于检测和量化体外培养的哺乳动物细胞中的细胞毒性效应。在用抗肿瘤药物阿霉素处理之前和之后，已获取描绘同一多细胞球体（实体瘤模型）中微观存活和非存活区域的图像。检测到存活边缘内部区域活力的丧失。对于常规用于测量活力的方法，如组织学检查，不可能在同一完整生物对象中对存活和非存活细胞定位的时间进程变化进行这样的映射。已经评估了球体EPRI高精度和高灵敏度所需的实验条件，并指出了该方法进一步发展的方向。
