[通过移植内皮祖细胞扩大缺血皮瓣存活面积的实验]
[Experiment of augmenting the survival areas of ischemic flap by transplanting endothelial progenitor cells].
作者信息
Yi Cheng-gang, Guo Shu-zhong, Zhang Lin-xi, Wu Jing-hua, Zhang Xi, Hu Qiang, Zhang Xu-dong, Zhou Qing-hong
机构信息
Department of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an, China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2005 Nov;21(6):429-32.
OBJECTIVE
To investigate the feasibility of transplanted endothelial progenitor cells to ischemic flap with increased neovascularization and augmented the survival areas.
METHODS
EPCs were isolated from human cord blood, cultured in vitro, identified by immunohistochemistry. Then EPCs were transplanted to ischemic flaps of 9 nude mice's back (experimental group), and 9 nude mice's back flaps was injected with M199(control group). And pedicle division time was 4 days after operation. CM-DiI was used to trace the transplanted cells. The blood perfusion of flaps was monitored by the laser Doppler flowetry, and the capillary density of flaps was detected by CD34 immunohistochemistry.
RESULTS
EPCs expressed cell markers CD34, KDR and CD133. Transplanted EPCs survived and was incorporated into the capillary networks in the ischemic flaps of nude mice. The percent of experimental group's flap survival area was (60.3 +/- 2.1)%, significantly higher than the control group[ (34.2 +/- 1.8)%, P < 0.05 ]. The blood perfusion, capillary density of flaps of experimental group was significantly higher than the control group (P < 0.05).
CONCLUSIONS
EPCs from human cord blood can increase ischemic flaps neovascularization and augment the survival areas.
目的
探讨移植内皮祖细胞促进缺血皮瓣血管新生及扩大存活面积的可行性。
方法
从人脐带血中分离内皮祖细胞，体外培养，免疫组织化学鉴定。然后将内皮祖细胞移植到9只裸鼠背部的缺血皮瓣（实验组），9只裸鼠背部皮瓣注射M199（对照组）。术后4天进行蒂部离断。用CM-DiI追踪移植细胞。用激光多普勒血流仪监测皮瓣的血流灌注，用CD34免疫组织化学检测皮瓣的毛细血管密度。
结果
内皮祖细胞表达细胞标志物CD34、KDR和CD133。移植的内皮祖细胞存活并整合到裸鼠缺血皮瓣的毛细血管网络中。实验组皮瓣存活面积百分比为(60.3±2.1)%，显著高于对照组[(34.2±1.8)%，P<0.05]。实验组皮瓣的血流灌注、毛细血管密度显著高于对照组（P<0.05）。
结论
人脐带血来源的内皮祖细胞可增加缺血皮瓣的血管新生并扩大存活面积。
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