[局部应用内皮祖细胞与血管内皮生长因子对促进预制皮瓣新生血管化作用的比较]

[Comparison of the effect of local administration of endothelial progenitor cells and VEGF on improving neovascularization in flap prefabrication].

作者信息

Zan Tao, Dong Ji-ying, Li Hua, Weng Rui, Yang Mei, Xie Yun, Li Qing-feng

机构信息

Department of Plastic and Reconstructive Surgery, the Ninth People' s Hospital of Shanghai Jiaotong University, Shanghai 200011, China.

出版信息

Zhonghua Zheng Xing Wai Ke Za Zhi. 2009 Nov;25(6):451-5.

PMID:20209939

Abstract

OBJECTIVE

To compare the effect of local administration of endothelial progenitor cells (EPCs) and VECF on improving neovascularization and augmenting the survival areas in a rat model of prefabricated flap.

METHODS

Prefabricated flaps were created by ligating the right femoral vascular pedicle and implanting it underneath the abdominal flap. The in vitro cultured EPCs (Group I , n=15) and VEGF protein (Group II , n=15) were injected subcutaneously around the implanted pedicle in experimental groups. PBS was injected in control group (Group Ill , n=15). 4 weeks later, the abdominal island flap based solely on the implanted vessels was elevated and sutured back. Then flap viability and numbers of capillary were evaluated on day 7.

RESULTS

There was more statistically significant augmentation of flap survival [(87.26 +/- 10.13) % versus (66.13 +/- 9.9)% and (55.59 +/- 13.06)%, P < 0.001], a higher capillary density (38.67 +/- 9.52 versus 25.83 +/- 6.33 and 26.5 +/- 5.61 capillary/mm2 , P < 0.05) in EPCs group than in the other two groups.

CONCLUSIONS

EPCs are superior to VEGF in improving neovascularization during flap prefabrication. Local transplantation of bone marrow-derived EPCs may be a useful strategy for augmentation of the survival areas of prefabricated flaps.

摘要

目的

比较局部应用内皮祖细胞（EPCs）和血管内皮生长因子（VEGF）对预制皮瓣大鼠模型新生血管形成及存活面积增加的影响。

方法

通过结扎右侧股血管蒂并将其植入腹部皮瓣下方制作预制皮瓣。实验组在植入的血管蒂周围皮下注射体外培养的EPCs（I组，n = 15）和VEGF蛋白（II组，n = 15）。对照组（III组，n = 15）注射磷酸盐缓冲液（PBS）。4周后，仅基于植入血管的腹部岛状皮瓣被掀起并缝合回原位。然后在第7天评估皮瓣活力和毛细血管数量。

结果

EPCs组皮瓣存活率的增加在统计学上更显著[（87.26 ± 10.13）% 对比（66.13 ± 9.9）% 和（55.59 ± 13.06）%，P < 0.001]，毛细血管密度更高（38.67 ± 9.52 对比 25.83 ± 6.33 和 26.5 ± 5.61 根毛细血管/mm2，P < 0.05），优于其他两组。