Fan Shu-Qiang, Cai Jing-Long, Qin Li-Yan, Wang Zhi-Hong, Liu Zhen-Zhong, Sun Ming-Liang
Department of Burns and Plastic Surgery, The First Hospital of Xiamen Affiliated to Fujian Medical University, Fujian Province, Xiamen City, China.
Ann Plast Surg. 2008 Mar;60(3):299-305. doi: 10.1097/SAP.0b013e318061d310.
Heparin affects both dermal fibroblast proliferation and collagen and may mediate these effects by altering the levels of transforming growth factor-beta1 (TGF-beta1) production and TGF-beta1 mRNA expression as a wound healing modulator. The purpose of this study is to probe the effect of heparin on TGF-beta1 and TGF-beta1 mRNA production by human normal skin and hyperplastic scar fibroblasts. This research investigates the effect of heparin on TGF-beta1 and TGF-beta1 mRNA production by human normal skin and hyperplastic scar fibroblasts with exposure to 0 microg/mL, 100 microg/mL, 300 microg/mL, or 600 microg/mL heparin for 24, 48, 72, or 96 hours in a serum-free in vitro model. Levels of TGF-beta1 in the supernatants and TGF-beta1 mRNA expression of fibroblasts were determined by enzyme-linked immunosorbent assay (ELISA) and real time RT-PCR, respectively. Heparin (300 microg/mL and 600 microg/mL) stimulated TGF-beta1 production by normal skin (26% to 83%) and hyperplastic scar fibroblasts (63% to 85%), with statistical significance (P < 0.05) at various time points. Heparin (300 microg/mL and 600 microg/mL) also stimulated TGF-beta1 mRNA expression by normal skin (12% to 53%) and hyperplastic scar fibroblasts (33% to 52%), with statistical significance (P < 0.05) at various time points. These effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastic scar formation and wound healing in vivo.
肝素会影响真皮成纤维细胞的增殖以及胶原蛋白，并且可能通过改变转化生长因子-β1（TGF-β1）的产生水平和TGF-β1 mRNA表达，作为一种伤口愈合调节剂来介导这些作用。本研究的目的是探究肝素对人正常皮肤和增生性瘢痕成纤维细胞产生TGF-β1及TGF-β1 mRNA的影响。本研究在无血清体外模型中，研究了人正常皮肤和增生性瘢痕成纤维细胞在暴露于0微克/毫升、100微克/毫升、300微克/毫升或600微克/毫升肝素24、48、72或96小时后，肝素对TGF-β1及TGF-β1 mRNA产生的影响。分别通过酶联免疫吸附测定（ELISA）和实时逆转录聚合酶链反应（RT-PCR）测定上清液中TGF-β1的水平和成纤维细胞中TGF-β1 mRNA的表达。肝素（300微克/毫升和600微克/毫升）刺激正常皮肤（26%至83%）和增生性瘢痕成纤维细胞（63%至85%）产生TGF-β1，在各个时间点均具有统计学意义（P<0.05）。肝素（300微克/毫升和600微克/毫升）还刺激正常皮肤（12%至53%）和增生性瘢痕成纤维细胞（33%至52%）表达TGF-β1 mRNA，在各个时间点均具有统计学意义（P<0.05）。肝素对正常皮肤和增生性瘢痕成纤维细胞的这些作用可能对体内增生性瘢痕形成和伤口愈合有影响。
