Engel Amber R, Vasconcelos Pedro F C, McArthur Monica A, Barrett Alan D T
Department of Pathology, The University of Texas Medical Branch, Galveston, TX 77555-0609, USA.
Vaccine. 2006 Apr 5;24(15):2803-9. doi: 10.1016/j.vaccine.2006.01.009. Epub 2006 Jan 18.
Although the live attenuated yellow fever (YF) 17D vaccine is considered to be one of the safest vaccines in the world today, several cases of disease associated with administration of the vaccine have been reported, including YF vaccine-associated viscerotropic disease (YF-VAVD), which was first described in 1996. All YF-VAVD isolates sequenced to date have shown very little genomic change when compared to their parental vaccine strains. In this study, we report the characterization of an isolate, BeH291597 (Brazil75), from a 1975 fatal case of YF-VAVD in Brazil. Comparison of Brazil75 with the genomic sequence of the parental 17DD vaccine strain revealed two amino acid substitutions (at positions M-49 and NS4B-240) that were unique to Brazil75. Although still a rare occurrence, this isolate suggests that YF-VAVD has been present much longer than previously recognized.
尽管减毒活黄热病（YF）17D疫苗被认为是当今世界上最安全的疫苗之一，但仍有几例与该疫苗接种相关的疾病报告，包括1996年首次描述的黄热病疫苗相关内脏型疾病（YF-VAVD）。迄今为止测序的所有YF-VAVD分离株与它们的亲代疫苗株相比，基因组变化都非常小。在本研究中，我们报告了一株来自1975年巴西YF-VAVD死亡病例的分离株BeH291597（Brazil75）的特征。将Brazil75与亲代17DD疫苗株的基因组序列进行比较，发现了两个氨基酸替换（位于M-49和NS4B-240位置），这是Brazil75所特有的。尽管仍然罕见，但该分离株表明YF-VAVD存在的时间比以前认为的要长得多。
