Cecchi L, Malaspina P
Centro per lo Studio Tecnologico, Bromatologico e Microbiologico del Latte, CNR, Milano, Italy.
Anal Biochem. 1991 Jan;192(1):219-21. doi: 10.1016/0003-2697(91)90211-b.
A simple and fast method for the separation of D- and L-lactic acid enantiomers by thin-layer chromatographic silica gel precoated plates impregnated with Cu(II) is proposed. The two lactic acid enantiomers, dissolved in acetone, are deposed on these plates. D- and L-lactic acids form complexes with Cu(II), with different Rf values, suitable for radiochemical measurements. The real separation was confirmed by enzymatic tests carried out on spots of D and L forms scraped from the chromatographic plates. Total radioactivity of 14C-labeled D- and L-lactic acids was recovered. A Cu/lactic acid complex is evidenced.
提出了一种通过浸渍有Cu(II)的薄层色谱硅胶预制板分离D-和L-乳酸对映体的简单快速方法。将溶解在丙酮中的两种乳酸对映体点样在这些板上。D-和L-乳酸与Cu(II)形成络合物，其比移值不同，适用于放射化学测量。通过对从色谱板上刮下的D型和L型斑点进行酶促试验，证实了实际的分离效果。回收了14C标记的D-和L-乳酸的总放射性。证明了铜/乳酸络合物的存在。
