Yan Li, Inamori Ryuhei, Gui Ping, Xu Kai-qin, Kong Hai-nan, Matsumura Masatoshi, Inamori Yuhei
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
J Environ Sci (China). 2005;17(6):993-7.
A molecular biology method, fluorescent in situ hybridization (FISH), in which the pre-treatment was improved in allusion to the media of the constructed wetlands (CW), e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria (AOB) quantity and the relation with oxidation-reduction potential (ORP) in the Typha latifolia constructed wetlands under three different loadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.
采用一种分子生物学方法——荧光原位杂交（FISH），针对人工湿地（CW）的介质（如土壤和沙砾）对预处理进行了改进，用于研究5月至9月夏季三种不同负荷下宽叶香蒲人工湿地中氨氧化细菌（AOB）数量的垂直分布特征及其与氧化还原电位（ORP）的关系。结果表明，宽叶香蒲人工湿地中AOB数量随垂直深度增加而减少。然而，根区AOB数量是对照数量的2至4倍。此外，发现根际的ORP高于其他区域，这表明在中国太湖农村地区使用模拟面源污水和小镇混合污水时，宽叶香蒲人工湿地在夏季处于好氧状态。
