Duggin Iain G, Bell Stephen D
MRC Cancer Cell Unit, Hutchison/Medical Research Council Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom.
J Biol Chem. 2006 Jun 2;281(22):15029-32. doi: 10.1074/jbc.R500029200. Epub 2006 Feb 8.
In the three domains of life, the archaea, bacteria, and eukarya, there are two general lineages of DNA replication proteins: the bacterial and the eukaryal/archaeal lineages. The hyperthermophilic archaeon Sulfolobus solfataricus provides an attractive model for biochemical study of DNA replication. Its relative simplicity in both genomic and biochemical contexts, together with high protein thermostability, has already provided insight into the function of the more complex yet homologous molecules of the eukaryotic domain. Here, we provide an overview of recent insights into the functioning of the chromosome replication machinery of S. solfataricus, focusing on some of the relatively well characterized core components that act at the DNA replication fork.
在生命的三个域，即古菌、细菌和真核生物中，DNA复制蛋白存在两个主要谱系：细菌谱系和真核生物/古菌谱系。嗜热古菌嗜热栖热菌为DNA复制的生化研究提供了一个有吸引力的模型。它在基因组和生化环境方面相对简单，再加上蛋白质具有较高的热稳定性，这已经为了解真核域中更复杂但同源的分子的功能提供了线索。在这里，我们概述了对嗜热栖热菌染色体复制机制功能的最新见解，重点关注一些在DNA复制叉处起作用且特征相对明确的核心成分。
