二乙氨基乙基葡聚糖对小鼠胚胎培养物中鼠肉瘤（莫洛尼氏）-白血病病毒复合物复制的影响（38577）

Effect of diethylaminoethyl-dextran on the replication of a murine sarcoma (Moloney)-leukemia virus complex in mouse embryo cultures (38577).

作者信息

Hirschman S Z, Funke V

出版信息

Proc Soc Exp Biol Med. 1975 Feb;148(2):527-31. doi: 10.3181/00379727-148-38577.

Abstract

Isolates of a sarcoma (Moloney)-leukemia virus complex prepared as cell-free extracts from mouse tumors showed no enhancement in infectivity by DEAE-D either in the sarcoma moiety measured by focus formation or in the leukemia moiety measured by XC cell assay. The sarcoma moiety was not enhanced by DEAE-D in MEM and modified McCoy's 5a media or when varying amounts of FCS and glutamine were included in the media. Progressive enhancement of viral infectivity by DEAE-D was found when the viral preparations were passaged serially in MEF cells. DEAE-D ALSO ENHANCED TUMOR FORMATION IN VIVO BY TISSUE CULTURE PASSAGED VIRUS.

摘要

从小鼠肿瘤制备的无细胞提取物形式的肉瘤（莫洛尼）-白血病病毒复合物分离株，无论是通过集落形成测量的肉瘤部分，还是通过XC细胞测定测量的白血病部分，用二乙氨基乙基葡聚糖（DEAE-D）处理后感染性均未增强。在MEM和改良的 McCoy's 5a培养基中，或当培养基中含有不同量的胎牛血清（FCS）和谷氨酰胺时，DEAE-D均未增强肉瘤部分的感染性。当病毒制剂在小鼠胚胎成纤维细胞（MEF细胞）中连续传代时，发现DEAE-D可逐渐增强病毒的感染性。DEAE-D还增强了经组织培养传代的病毒在体内的致瘤性。