Chuang L Y, Tsai J H, Yeh Y C, Chang C C, Yeh H W, Guh J Y, Tsai J F
Department of Biochemistry, Kaohsiung Medical College, Taiwan, Republic of China.
Hepatology. 1991 Jun;13(6):1112-6.
To characterize epidermal growth factor-related transforming growth factors in the urine of patients with hepatocellular carcinoma, gel filtration with Bio-Gel P-30 was performed in seven hepatocellular carcinoma patients and seven sex-matched and age-matched healthy controls. Distinct profiles of soft agar growth assay in the hepatocellular carcinoma patients and the normal controls were seen. Three peaks (A, B and C) in the urine were examined. Peak C in most hepatocellular carcinoma patients was higher than that in healthy controls. Similar profiles were detected with epidermal growth factor radioreceptor assay and cellular DNA synthesis assay. This result might indicate that transforming growth factors with low molecular weight were found in the urine of hepatocellular carcinoma patients. An exceptional HCC patient had an additional peak (A') that corresponded to the high molecular weight protein. We concluded that there were transforming growth factors with functional activity in the urine of patients with hepatocellular carcinoma.
为了鉴定肝细胞癌患者尿液中与表皮生长因子相关的转化生长因子，对7例肝细胞癌患者以及7例性别和年龄匹配的健康对照者进行了Bio-Gel P-30凝胶过滤。在肝细胞癌患者和正常对照者中观察到了软琼脂生长试验的不同图谱。检测了尿液中的三个峰（A、B和C）。大多数肝细胞癌患者的C峰高于健康对照者。通过表皮生长因子放射受体测定和细胞DNA合成测定检测到了相似的图谱。这一结果可能表明在肝细胞癌患者的尿液中发现了低分子量的转化生长因子。一名特殊的肝癌患者有一个额外的峰（A'），它对应于高分子量蛋白质。我们得出结论，肝细胞癌患者的尿液中存在具有功能活性的转化生长因子。
