Watanabe T, Yasunari K, Nakamura M, Maeda K
Department of Medicine and Cardiology, Osaka City University Medical School, Asahimachi, Abeno, Japan.
J Hum Hypertens. 2006 May;20(5):336-40. doi: 10.1038/sj.jhh.1001990.
Carotid artery intima-media thickness (IMT) is a widely accepted index for assessing atherosclerosis, and is known to be a risk indicator for cardiovascular and cerebrovascular events. Oxidative stress and inflammation are also known to play critical roles in the pathogenesis of vascular events. We studied the association between IMT and inflammatory markers, such as oxidative stress in polymorphonuclear leukocytes (PMNs) and mononuclear cells (MNCs) in 156 patients with essential hypertension. Reactive oxygen species (ROS) formation by PMNs and MNCs was measured by gated flow cytometry. CRP and traditional risk factors, such as age, gender, body mass index, hemoglobin A(1c), and total cholesterol were also measured. The subjects were divided into a plaque group (max-IMT>or=1.1 mm, n=40), and a nonplaque group (max-IMT<1.1 mm, n=116). ROS formation by MNCs was significantly increased in the plaque group when compared with the nonplaque group (P<0.0001). Multiple regression analysis revealed a significant correlation between IMT and ROS formation by MNCs (r=0.407, P<0.0001), or CRP (r=0.216, P=0.0029) or hemoglobinA1c (r=0.158, P=0.0270) or age (r=0.157, P=0.0447). No significant correlation was observed between IMT and ROS formation by PMNs. These results suggest that carotid artery IMT may be affected by increased ROS formation by MNCs, and that increased ROS formation by MNCs may be related to the development of atherosclerosis. We propose that ROS formation by MNCs is a marker for prediction of carotid atherosclerosis.
颈动脉内膜中层厚度（IMT）是评估动脉粥样硬化的一个广泛接受的指标，并且已知是心血管和脑血管事件的风险指标。氧化应激和炎症在血管事件的发病机制中也起着关键作用。我们研究了156例原发性高血压患者的IMT与炎症标志物之间的关联，这些炎症标志物包括多形核白细胞（PMN）和单核细胞（MNC）中的氧化应激。通过门控流式细胞术测量PMN和MNC产生的活性氧（ROS）。还测量了CRP以及年龄、性别、体重指数、糖化血红蛋白A1c和总胆固醇等传统风险因素。将受试者分为斑块组（最大IMT≥1.1mm，n = 40）和非斑块组（最大IMT＜1.1mm，n = 116）。与非斑块组相比，斑块组中MNC产生的ROS显著增加（P＜0.0001）。多元回归分析显示IMT与MNC产生的ROS（r = 0.407，P＜0.0001）、CRP（r = 0.216，P = 0.0029）、糖化血红蛋白A1c（r = 0.158，P = 0.0270）或年龄（r = 0.157，P = 0.0447）之间存在显著相关性。未观察到IMT与PMN产生的ROS之间存在显著相关性。这些结果表明，颈动脉IMT可能受MNC产生的ROS增加的影响，并且MNC产生的ROS增加可能与动脉粥样硬化的发展有关。我们提出，MNC产生的ROS是预测颈动脉粥样硬化的一个标志物。
