Wang Li-Hong, Zhang Lin, Chen Huan-Lin
College of Materials Sci. & Chem. Eng., Zhejiang University, Hangzhou, 310027, China.
Sheng Wu Gong Cheng Xue Bao. 2005 Nov;21(6):954-9.
A Triazophos-degrading strain, Klebsiella sp. E6, was identified by soil enrichment technology from the soil sampled from the vicinity of a factory manufacturing Triazophos (TAP). The nutrient requirement of the strain is simple. It can use TAP as the sole sources of carbon, nitrogen and phosphorus. Comparison of the degradation rates revealed that the strain degraded TAP most effectively when TAP was used as a sole nitrogen source. No inhibition effect occurred when TAP concentration was high as 1000 mg/L in the case of TAP was used as the sole nitrogen source. Analysis of the intermediates of TAP metabolism indicated that TAP is firstly hydrolyzed into 1-phenyl-3-hydroxy-1,2,4-triazole and O,O-diethyl phosphorothioic acid. 1-phenyl-3-hydroxy-1,2,4-triazole was further mineralized into inorganic compounds. A degradation pathway of TAP was proposed. The experiment results demonstrated that the strain has potential in biodegradation of TAP pollutions.
通过土壤富集技术，从生产三唑磷（TAP）的工厂附近采集的土壤中分离出一株三唑磷降解菌，即克雷伯氏菌属E6菌株。该菌株对营养的需求很简单，它能够利用三唑磷作为唯一的碳、氮和磷源。降解率比较结果表明，当三唑磷作为唯一氮源时，该菌株对三唑磷的降解效果最佳。在三唑磷作为唯一氮源的情况下，当三唑磷浓度高达1000mg/L时，未出现抑制作用。对三唑磷代谢中间产物的分析表明，三唑磷首先水解为1-苯基-3-羟基-1,2,4-三唑和O,O-二乙基硫代磷酸。1-苯基-3-羟基-1,2,4-三唑进一步矿化为无机化合物。据此提出了三唑磷的降解途径。实验结果表明，该菌株在生物降解三唑磷污染方面具有潜力。
