β-半乳糖苷酶基因工程乳酸乳球菌对乳糖所致细胞毒性的缓解作用
[Relief effect of beta-galactosidase genetically engineered lactococcus lactis on the cell toxicity caused by lactose].
作者信息
Liu Xiang, Lü Xiao-ying, Yu Qian, Zhang Chao-wu
机构信息
Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):52-4.
OBJECTIVE
To assess the relief effect of beta-galactosidase genetically engineered Lactococcus lactis on the cell toxicity caused by lactose in vitro.
METHODS
An in vitro toxic Caco-2 cell model caused by lactose was established to evaluate the relief effect of beta-galactosidase genetically engineered Lactococcus lactis. Cell morphological parameters and proliferation activity parameter were used.
RESULTS
The in vitro toxic Caco-2 cell model caused by lactose was successfully established; the genetically engineered Lactococcus lactis constructed in the authors' laboratory could enable the Caco-2 cell to have normal appearance with the presence of lactose and could improve the proliferation activity with the presence of high concentration of lactose (P < 0.01).
CONCLUSION
The beta-galactosidase genetically engineered Lactococcus lactis has significant relief effect on the cell toxicity caused by lactose in vitro, which lays a foundation for food-grade alternation of this bacterium.
目的
评估β-半乳糖苷酶基因工程乳酸乳球菌对乳糖体外细胞毒性的缓解作用。
方法
建立乳糖诱导的体外毒性Caco-2细胞模型，以评估β-半乳糖苷酶基因工程乳酸乳球菌的缓解作用。采用细胞形态学参数和增殖活性参数。
结果
成功建立了乳糖诱导的体外毒性Caco-2细胞模型；作者实验室构建的基因工程乳酸乳球菌可使Caco-2细胞在乳糖存在时外观正常，并在高浓度乳糖存在时提高增殖活性（P<0.01）。
结论
β-半乳糖苷酶基因工程乳酸乳球菌对乳糖体外细胞毒性具有显著缓解作用，为该菌的食品级改造奠定了基础。
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