[人胶质瘤干细胞的长期培养与分化]
[Long-term culture and differentiation of human glioma stem cells ].
作者信息
Yang Li-ye, Wang Chao-yang, Zheng Jia-kun, Wen Jian-cheng, Li Wen-yu, Huang Xiao-ping
机构信息
Department of Neurosurgery, Chaozhou Central Hospital, Chaozhou 521021, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):141-4.
OBJECTIVE
To explore the culture and subculture conditions for glioma stem cells(GSCs) and to investigate the differentiation potential of GSCs.
METHODS
The cells from human glioma were mechanically dissociated. Cells were cultured in N2 or B27 medium with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and they were identified by immunocytochemistry.
RESULTS
Glioma stem cells from human glioma have been successfully cultured. They formed typical neurospheres in suspension, and they could be cultured and passaged steadily in vitro. The majorities of the cells expressed vimentin and nestin, which were the markers for neural stem cells. They could differentiate into neurons and astrocytes, and express glial fibrillary acidic protein and beta III-tubulin respectively.
CONCLUSION
Human glioma stem cells could be cultured from gliomas in vitro, and they could differentiate into neurons and astrocytes, thus providing a basis for further studies.
目的
探索胶质瘤干细胞（GSCs）的培养及亚培养条件，并研究GSCs的分化潜能。
方法
将人胶质瘤细胞进行机械解离。细胞在含有碱性成纤维细胞生长因子（bFGF）和表皮生长因子（EGF）的N2或B27培养基中培养，并用免疫细胞化学法进行鉴定。
结果
已成功培养出人胶质瘤干细胞。它们在悬浮状态下形成典型的神经球，且能在体外稳定培养和传代。大多数细胞表达波形蛋白和巢蛋白，这是神经干细胞的标志物。它们可分化为神经元和星形胶质细胞，并分别表达胶质纤维酸性蛋白和βⅢ微管蛋白。
结论
人胶质瘤干细胞可在体外从胶质瘤中培养出来，且能分化为神经元和星形胶质细胞，从而为进一步研究提供了基础。
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