Dolezelová E, Zurovec M, Böhmová M, Sehnal F
Institute of Entomology of the Academy of Sciences and Faculty of Biological Sciences of the University of South Bohemia, Ceské Budejovice, Czech Republic.
Insect Mol Biol. 2006 Feb;15(1):25-32. doi: 10.1111/j.1365-2583.2006.00604.x.
The enzyme glucose-6-phosphate dehydrogenase (G6PD) of the bark beetle Ips typographus is derived from a gene that includes eight exons and spans over 7100 nucleotides (nt). By means of two transcription starts, the gene generates two mRNA isoforms that are present in similar amounts in the larvae, pupae and adults. The A isoform includes exon IA of 115 nt, which is followed by intron 1a extending to position 3457 of the gene. The B mRNA isoform begins with exon IB (100 nt) that occupies positions 3291-3390 within the 1a intron. Exons II to VII are included in both mRNA isoforms. The gene contains 31.6% (36.5% in the translated region) of the GC nucleotides. Two transcription starts and the exon/intron organization distinguish bark beetle G6PD from the homologous genes known in other insects. Two enzyme variants were detected in the protein extracts of individual bark beetles but their relationship to the A and B mRNA isoforms is uncertain.
欧洲云杉八齿小蠹的葡萄糖-6-磷酸脱氢酶（G6PD）由一个包含8个外显子、跨越7100多个核苷酸（nt）的基因产生。通过两个转录起始位点，该基因产生两种mRNA异构体，它们在幼虫、蛹和成虫中的含量相似。A异构体包含115 nt的外显子IA，其后是延伸至基因第3457位的内含子1a。B mRNA异构体起始于外显子IB（100 nt），它位于1a内含子内的第3291 - 3390位。外显子II至VII包含在两种mRNA异构体中。该基因的GC核苷酸含量为31.6%（翻译区域为36.5%）。两个转录起始位点和外显子/内含子结构使欧洲云杉八齿小蠹的G6PD与其他昆虫中已知的同源基因有所不同。在单个欧洲云杉八齿小蠹的蛋白质提取物中检测到两种酶变体，但它们与A和B mRNA异构体的关系尚不确定。
