Cardoso Paula Cristina Marques, Caldeira Roberta Lima, Lovato Maria Bernadete, Coelho Paulo Marcos Zech, Berne Maria Elisabeth Aires, Müller Gertrud, Carvalho Omar dos Santos
Oswaldo Cruz Foundation, Laboratório de Helmintoses Intestinais, Centro de Pesquisas René Rachou-Fiocruz, Avenida Augusto de Lima 1715, Barro Preto MG 30190-002, Belo Horizonte, Minas Gerais, Brazil.
Acta Trop. 2006 Mar;97(3):339-45. doi: 10.1016/j.actatropica.2006.01.003. Epub 2006 Feb 15.
In Brazil, Lymnaea columella is the most important intermediate host of Fasciola hepatica, the etiological agent of fasciolosis, which is a parasitic disease of veterinarian and human importance. Random amplified polymorphic DNA (RAPD) was used to investigate the genetic variability within and among nine Brazilian populations of L. columella comprising 205 individuals. A number of four primers were used for analysis of molecular variance (AMOVA). Out of 83 RAPD markers, 63 (76%) were polymorphic and revealed 119 unique RAPD profiles. The levels of genetic variability found in the populations were low and most of the genetic variation was interpopulational (81.6%) when compared to intrapopulational variability (18.4%). These results are in accordance with the dynamics and distribution of the populations analyzed.
在巴西，小椎实螺是肝片吸虫最重要的中间宿主，肝片吸虫是片形吸虫病的病原体，这是一种对兽医和人类都很重要的寄生虫病。随机扩增多态性DNA（RAPD）被用于研究由205个个体组成的9个巴西小椎实螺种群内部和种群之间的遗传变异性。使用了4种引物进行分子方差分析（AMOVA）。在83个RAPD标记中，63个（76%）是多态性的，并揭示了119个独特的RAPD图谱。与种群内变异性（18.4%）相比，所发现的种群遗传变异性水平较低，且大部分遗传变异是种群间的（81.6%）。这些结果与所分析种群的动态和分布情况相符。
