Liegeois Florian, Courgnaud Valerie, Switzer William M, Murphy Hayley W, Loul Severin, Aghokeng Avelin, Pourrut Xavier, Mpoudi-Ngole Eitel, Delaporte Eric, Peeters Martine
UMR145, Institut de Recherche pour le Developpement, University of Montpellier I, Montpellier, France.
Virology. 2006 May 25;349(1):55-65. doi: 10.1016/j.virol.2006.01.011. Epub 2006 Feb 8.
Simian immunodeficiency viruses (SIVs) are found in an extensive number of African primates, and humans continue to be exposed to these viruses by hunting and handling of primate bushmeat and following occupational exposures to captive nonhuman primates. Here, we report the molecular characterization of a new SIV lineage, SIVtal, from wild-caught and captive talapoin monkeys (Miopithecus ogouensis) from Cameroon and U.S. zoos, respectively. Phylogenetic tree analyses of a small fragment in the pol gene indicated that all SIVtal strains clustered together forming a single species-specific lineage. Full-length sequence analysis for two strains, SIVtal-00CM266 and SIVtal-01CM8023, from wild-caught animals in Cameroon confirmed that SIVtal was distinct from all primate lentiviruses isolated so far and represents a new SIV lineage. Phylogenetic analyses in different viral genes showed a significant clustering of the SIVtal lineage with the Cercopithecus-specific SIVs. In addition, SIVtal and Cercopithecus-specific SIVs share functional motifs in Gag and Env that distinguish them from other primate lentiviruses. Like SIVsyk and SIVdeb, a vpu gene homologue was also absent in SIVtal. Although northern talapoins belong to the Miopithecus genus, their SIVs belong to the Cercopithecus SIV lineage, suggesting evolution from a common ancestor or cross-species transmission between both primate genera.
猿猴免疫缺陷病毒（SIVs）在大量非洲灵长类动物中被发现，人类通过捕猎和处理灵长类丛林肉以及职业接触圈养的非人类灵长类动物，持续暴露于这些病毒。在此，我们报告了一种新的SIV谱系SIVtal的分子特征，该谱系分别来自喀麦隆野生捕获以及美国动物园圈养的长尾猴（Miopithecus ogouensis）。对pol基因中的一个小片段进行的系统发育树分析表明，所有SIVtal毒株聚集在一起，形成了一个单一的物种特异性谱系。对来自喀麦隆野生捕获动物的两个毒株SIVtal - 00CM266和SIVtal - 01CM8023进行的全长序列分析证实，SIVtal与迄今分离出的所有灵长类慢病毒不同，代表了一种新的SIV谱系。在不同病毒基因中进行的系统发育分析显示，SIVtal谱系与猕猴特异性SIVs有显著聚类。此外，SIVtal和猕猴特异性SIVs在Gag和Env中共享功能基序，这使它们与其他灵长类慢病毒区分开来。与SIVsyk和SIVdeb一样，SIVtal中也不存在vpu基因同源物。尽管北方长尾猴属于猕猴属，但其SIVs属于猕猴SIV谱系，这表明它们是从一个共同祖先进化而来，或者是这两个灵长类属之间发生了跨物种传播。
