Wu Yan-Jun, Zhang Shang-Long, Xie Ming, Chen Jun-Wei, Jiang Gui-Hua, Qin Yong-Hua, Qin Qiao-Ping
Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hongzhou 310029, China.
Yi Chuan. 2006 Jan;28(1):65-70.
Genetic transformation of peach immature cotyledons with its ACO antisense gene was studied by using particle bombardment method through Agrobacterium tumefaciens. Kanr shoots and Kanr plantlet were obtained. The plantet with ACO antisenes gene through Agrobacterium tumefaciens was obtained by micrografting technique and survived for nearly one month. The results of the PCR, PCR-southern, genomes southern hybridization analysis and GUS color reaction of some Kanr materials showed in some degree that the peach ACO antisens gene was integrated into peach genomes.
利用农杆菌介导的粒子轰击法研究了桃未成熟子叶的ACO反义基因遗传转化。获得了卡那霉素抗性芽和卡那霉素抗性植株。通过微嫁接技术获得了通过农杆菌介导的具有ACO反义基因的植株，并存活了近一个月。对一些卡那霉素抗性材料的PCR、PCR- Southern、基因组Southern杂交分析和GUS显色反应结果在一定程度上表明桃ACO反义基因已整合到桃基因组中。
