Matusek Tamás, Djiane Alexandre, Jankovics Ferenc, Brunner Damian, Mlodzik Marek, Mihály József
Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62, Hungary.
Development. 2006 Mar;133(5):957-66. doi: 10.1242/dev.02266.
Formins are involved in a wide range of cellular processes that require the remodeling of the actin cytoskeleton. Here, we have analyzed a novel Drosophila formin, belonging to the recently described DAAM subfamily. In contrast to previous assumptions, we show that DAAM plays no essential role in planar cell polarity signaling, but it has striking requirements in organizing apical actin cables that define the taenidial fold pattern of the tracheal cuticle. These observations provide evidence the first time that the function of the taenidial organization is to prevent the collapse of the tracheal tubes. Our results indicate that although DAAM is regulated by RhoA, it functions upstream or parallel to the non-receptor tyrosine kinases Src42A and Tec29 to organize the actin cytoskeleton and to determine the cuticle pattern of the Drosophila respiratory system.
formin蛋白参与了广泛的细胞过程，这些过程需要肌动蛋白细胞骨架的重塑。在这里，我们分析了一种新型的果蝇formin蛋白，它属于最近描述的DAAM亚家族。与之前的假设相反，我们发现DAAM在平面细胞极性信号传导中没有关键作用，但在组织顶端肌动蛋白索方面有显著需求，这些肌动蛋白索决定了气管角质层的环褶模式。这些观察首次提供了证据，表明环褶组织的功能是防止气管塌陷。我们的结果表明，尽管DAAM受RhoA调控，但它在非受体酪氨酸激酶Src42A和Tec29的上游或与其平行发挥作用，以组织肌动蛋白细胞骨架并确定果蝇呼吸系统的角质层模式。
