Gu Y, Lin Y C
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Res Commun Chem Pathol Pharmacol. 1991 Apr;72(1):27-38.
We have reported that gossypol, an antifertility agent, and gossypolone, a major metabolite of gossypol, inhibited steroidogenesis in bovine luteal cells in vitro. In the current study, the effects of these two compounds on steroidogenesis were examined in adrenocortical cells of young rats. ACTH-induced corticosterone secretion and intracellular adenosine monophosphate (cAMP) formation were significantly inhibited by gossypol at concentrations of 8.6 and 4.3 microM, respectively, and by gossypolone at concentrations of 8.6 and 4.3 microns, respectively. The present study demonstrated that both compounds inhibit ACTH-induced corticosterone synthesis and secretion in rat adrenocortical cell and this inhibition is, at least partially, attributed to the reduction of intracellular cAMP formation. Our data suggest that gossypol and its metabolite(s) probably affect steroidogenesis not only in gonads but also in adrenal glands by a common mean-reduction of intracellular cAMP.
我们曾报道，抗生育药物棉酚及其主要代谢产物棉酚酮在体外可抑制牛黄体细胞的类固醇生成。在本研究中，检测了这两种化合物对幼龄大鼠肾上腺皮质细胞类固醇生成的影响。棉酚在浓度分别为8.6微摩尔和4.3微摩尔时，以及棉酚酮在浓度分别为8.6微米和4.3微米时，均可显著抑制促肾上腺皮质激素（ACTH）诱导的皮质酮分泌和细胞内单磷酸腺苷（cAMP）生成。本研究表明，这两种化合物均可抑制大鼠肾上腺皮质细胞中ACTH诱导的皮质酮合成与分泌，且这种抑制作用至少部分归因于细胞内cAMP生成的减少。我们的数据表明，棉酚及其代谢产物可能通过共同降低细胞内cAMP的方式，不仅影响性腺中的类固醇生成，还影响肾上腺中的类固醇生成。
