Biegalke B J, Geballe A P
Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Virology. 1991 Jul;183(1):381-5. doi: 10.1016/0042-6822(91)90151-z.
Human cytomegalovirus (CMV) alters the growth and expression of human immunodeficiency virus-1 (HIV-1) in cell culture and may accelerate the course of AIDS in HIV-1 infected patients. CMV infection or the expression of the CMV immediate early genes has been shown to activate gene expression directed by the HIV-1 LTR. However, the cis-acting elements within the HIV-1 LTR that confer responsiveness to CMV have not been clearly delineated. We report on investigations in human fibroblasts designed to precisely map this signal. Our studies demonstrate that more than one nonoverlapping region of the HIV-1 promoter is capable of responding to CMV. Sequences 3' from -19(relative to the start of transcription) are dispensable for CMV responsiveness. We also show that in addition to immediate early region 2, immediate early region 1 is able to activate HIV-1 LTR-directed gene expression.
人巨细胞病毒（CMV）可在细胞培养中改变人类免疫缺陷病毒1型（HIV-1）的生长和表达，并可能加速HIV-1感染患者的艾滋病病程。CMV感染或CMV立即早期基因的表达已被证明可激活由HIV-1长末端重复序列（LTR）指导的基因表达。然而，HIV-1 LTR中赋予对CMV反应性的顺式作用元件尚未明确界定。我们报告了旨在精确绘制此信号的人类成纤维细胞研究。我们的研究表明，HIV-1启动子的多个非重叠区域能够对CMV作出反应。相对于转录起始点-19下游的序列对于CMV反应性是可有可无的。我们还表明，除了立即早期区域2外，立即早期区域1也能够激活HIV-1 LTR指导的基因表达。
