Ponglowhapan Suppawiwat, Chatdarong Kaywalee, Sirivaidyapong Sudson, Lohachit Chainarong
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Sciences, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
Theriogenology. 2006 Oct;66(6-7):1633-6. doi: 10.1016/j.theriogenology.2006.01.032. Epub 2006 Feb 10.
An experiment was conducted to investigate the freezing ability of canine epididymal spermatozoa after cool storage at 5 degrees C for 2 or 4 days. Spermatozoa were collected from the caudae epididymidis from 16 dogs. Total motility, plasma membrane integrity and acrosome integrity were evaluated immediately on harvesting, and after 2 and 4 days of storage at 5 degrees C, and at 0 and 2 h post-thaw at 37 degrees C. Sperm motility decreased significantly during cold storage, compared to freshly harvested spermatozoa (P < 0.001). Although there was no significant effect of pre-freeze storage time on post-thaw motility, there was a tendency towards decreased motility in spermatozoa that had been stored for 4 days, compared to spermatozoa that were frozen immediately after collection (P = 0.09). The number of post-thaw spermatozoa with an intact plasma membrane was decreased in spermatozoa cold-stored for 4 days (P < 0.001). There was no significant effect of pre-freeze storage time on the acrosomal status of post-thaw spermatozoa. In conclusion, canine epididymal spermatozoa were stored at 5 degrees C for up to 4 days without a clear detrimental effect on post-thaw motility and acrosome integrity, but storage may have decreased post-thaw motility. Results were, however, generally low.
进行了一项实验，以研究犬附睾精子在5℃冷藏2天或4天后的冷冻能力。从16只犬的附睾尾部采集精子。在采集后、5℃储存2天和4天后以及37℃解冻后0小时和2小时，立即评估精子的总活力、质膜完整性和顶体完整性。与新鲜采集的精子相比，冷藏期间精子活力显著下降（P<0.001）。尽管冷冻前储存时间对解冻后活力没有显著影响，但与采集后立即冷冻的精子相比，储存4天的精子活力有下降趋势（P = 0.09）。冷藏4天的精子解冻后质膜完整的精子数量减少（P<0.001）。冷冻前储存时间对解冻后精子的顶体状态没有显著影响。总之，犬附睾精子在5℃下储存长达4天，对解冻后活力和顶体完整性没有明显有害影响，但储存可能会降低解冻后活力。然而，结果普遍较低。
