利用Rab GTP酶研究正常及鞘脂贮积病成纤维细胞中的脂质转运。

Use of Rab GTPases to study lipid trafficking in normal and sphingolipid storage disease fibroblasts.

作者信息

Choudhury Amit, Marks David L, Pagano Richard E

出版信息

Methods Enzymol. 2005;403:166-82. doi: 10.1016/S0076-6879(05)03014-4.

Abstract

We describe methods for studying lipid transport in normal and sphingolipid storage disease fibroblasts. These techniques include endocytic assays with fluorescent sphingolipid analogs, expression of dominant negative (DN) Rab GTPases, and methods of manipulating cholesterol levels in intact cells and isolated cell membranes. These methods should be useful in future studies of lipid trafficking in normal and disease cell types.

摘要

我们描述了研究正常和成鞘脂贮积病成纤维细胞中脂质转运的方法。这些技术包括用荧光鞘脂类似物进行内吞测定、显性负性（DN）Rab GTP酶的表达，以及在完整细胞和分离细胞膜中操纵胆固醇水平的方法。这些方法在未来对正常和疾病细胞类型中脂质运输的研究中应该会有用。