乙醇对大量分离的脑细胞中多磷酸肌醇代谢的体外效应。
In vitro effect of ethanol on polyphosphoinositide metabolism in bulk isolated brain cells.
作者信息
George S, Parekh L J
机构信息
Department of Biochemistry, Faculty of Science, MS University of Baroda, India.
出版信息
Indian J Biochem Biophys. 1991 Feb;28(1):58-62.
Metabolism of polyphosphoinositide was studied in bulk isolated brain cells. Cells were isolated by a rapid method using mechanical disruption followed by molecular seiving and centrifugation. Incorporation of [32Pi]orthophosphate into phosphatidyl inositol-4-phosphate and phosphatidylinositol-4,5 bis-phosphate was optimum at 30 and 60 min, respectively, in the isolated cells. Breakdown studies showed maximum loss of 32Pi after 60 min. Addition of ethanol at and above 10 mM concentration in vitro significantly decreased the incorporation of 32Pi into both phosphatidylinositol-4-phosphate and phosphatidylinositol-4,5 bis-phosphate by the isolated cells. However, the spontaneous breakdown of polyphosphoinositide was not altered in the presence of ethanol in vitro.
在大量分离的脑细胞中研究了多磷酸肌醇的代谢。采用机械破碎、分子筛分离和离心的快速方法分离细胞。在分离的细胞中，[32P]正磷酸盐分别在30分钟和60分钟时最适宜掺入磷脂酰肌醇-4-磷酸和磷脂酰肌醇-4,5-二磷酸。分解研究表明，60分钟后32P的损失最大。体外添加浓度为10 mM及以上的乙醇会显著降低分离细胞将32P掺入磷脂酰肌醇-4-磷酸和磷脂酰肌醇-4,5-二磷酸的能力。然而，体外存在乙醇时，多磷酸肌醇的自发分解没有改变。
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