Heidel J R, Taylor S M, Silflow R M, Laegreid W W, Leid R W
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164-7040.
Inflammation. 1991 Feb;15(1):31-42. doi: 10.1007/BF00917907.
Leukotriene B4 (LTB4) and zymosan-activated plasma (ZAP) were each instilled into the lungs of steers to elicit alveolar neutrophils for subsequent functional analysis. Prior to instillation of either agent, bronchoalveolar lavage cell populations consisted of 95.8 +/- 0.4% macrophages (mean +/- SEM). Four hours after instillation of LTB4 or ZAP, the lavage cell populations consisted of 75.0 +/- 8.8% and 90.7 +/- 0.7% neutrophils, respectively. Alveolar neutrophils elicited with LTB4 and stimulated with the calcium ionophore A23187 released diminished amounts of LTB4 and increased amounts of 5-hydroxyeicosatetraenoic acid (5-HETE) as compared to circulating neutrophils. Release of superoxide anion was decreased for LTB4-elicited alveolar neutrophils as compared to circulating cells, while bacterial killing was unchanged. ZAP-elicited alveolar neutrophils released diminished amounts of LTB4 when stimulated with A23187 as compared to circulating neutrophils. There were no differences observed in 5-HETE levels between the two cell populations. In addition, release of superoxide anion was diminished among ZAP-elicited alveolar cells, while bacterial killing was unchanged. Incubation of circulating neutrophils with LTB4 did not influence the release of arachidonate metabolites, superoxide anion, or bacterial killing. However, incubation of circulating neutrophils with ZAP, followed by A23187 resulted in a reduction in the release of LTB4, as compared to control cells. Prior exposure to ZAP did not influence the release of superoxide anion or bacterial killing by the circulating neutrophils.
将白三烯B4（LTB4）和酵母聚糖激活血浆（ZAP）分别注入阉牛肺部，以引出肺泡中性粒细胞用于后续功能分析。在注入任何一种试剂之前，支气管肺泡灌洗细胞群由95.8±0.4%的巨噬细胞组成（平均值±标准误）。注入LTB4或ZAP后4小时，灌洗细胞群分别由75.0±8.8%和90.7±0.7%的中性粒细胞组成。与循环中的中性粒细胞相比，用LTB4引出并用钙离子载体A23187刺激的肺泡中性粒细胞释放的LTB4量减少，而5-羟基二十碳四烯酸（5-HETE）量增加。与循环细胞相比，LTB4引出的肺泡中性粒细胞超氧阴离子的释放减少，而细菌杀伤能力未变。与循环中的中性粒细胞相比，用A23187刺激时，ZAP引出的肺泡中性粒细胞释放的LTB4量减少。在这两种细胞群之间，未观察到5-HETE水平有差异。此外，ZAP引出的肺泡细胞中超氧阴离子的释放减少，而细菌杀伤能力未变。用LTB4孵育循环中的中性粒细胞不会影响花生四烯酸代谢产物、超氧阴离子的释放或细菌杀伤能力。然而，与对照细胞相比，用ZAP孵育循环中的中性粒细胞，随后用A23187处理，导致LTB4的释放减少。预先暴露于ZAP不会影响循环中的中性粒细胞超氧阴离子的释放或细菌杀伤能力。
