在酚类抗氧化剂存在的情况下，辣根过氧化物酶可降解脂质氢过氧化物并抑制多不饱和脂肪酸的脂质过氧化。

Horseradish peroxidase degrades lipid hydroperoxides and suppresses lipid peroxidation of polyunsaturated fatty acids in the presence of phenolic antioxidants.

作者信息

Shirasaka Norifumi, Ohnishi Hiromichi, Sato Keiko, Miyamoto Rie, Terashita Takao, Yoshizumi Hajime

机构信息

Department of Applied Biological Chemistry, Faculty of Agriculture, Kin-ki University, Nara, Japan.

出版信息

J Biosci Bioeng. 2005 Dec;100(6):653-6. doi: 10.1263/jbb.100.653.

DOI:10.1263/jbb.100.653

PMID:16473775

Abstract

Linoleic acid hydroperoxide (LAOOH) was effectively degraded by horseradish peroxidase (HRP) in the presence of quercetin. Several natural phenolic antioxidants, such as quercetin, capsaicin, and alpha-tocopherol, acted as good hydrogen donors in the peroxidase reaction that occurs during lipid hydroperoxide degradation. However, glutathione, which is a non-phenolic antioxidant that acts as a hydrogen donor for glutathione peroxidase, could not suppress lipid peroxidation in the presence of HRP. Lipid hydroperoxides generated from eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were also degraded with HRP in the presence of quercetin, and oxidative decomposition of DHA was suppressed by this reaction.

摘要

在槲皮素存在的情况下，辣根过氧化物酶（HRP）能有效降解亚油酸氢过氧化物（LAOOH）。几种天然酚类抗氧化剂，如槲皮素、辣椒素和α-生育酚，在脂质氢过氧化物降解过程中发生的过氧化物酶反应中作为良好的氢供体。然而，谷胱甘肽作为谷胱甘肽过氧化物酶的氢供体的非酚类抗氧化剂，在HRP存在时不能抑制脂质过氧化。二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）产生的脂质氢过氧化物在槲皮素存在的情况下也能被HRP降解，并且该反应抑制了DHA的氧化分解。