Norris B C, Pantoja C V, Chiang L C, Concha J B, Neumann V R, Ferrada O V
Department of Physiology, Faculty of Biological and Natural Sciences, University of Concepción, Chile.
J Ethnopharmacol. 1991 Mar;31(3):309-18. doi: 10.1016/0378-8741(91)90016-7.
The effect of an aqueous fraction from the bulbs of Allium sativum (GE) was investigated in toad skin. When added to the inner (serosal) solution, GE caused a maximal reversible reduction of the transepithelial potential difference and short circuit current of 38% and 45%, respectively. When added to the outer (mucosal) solution, the effect was only partially reversible. Isaacson's amiloride test showed that GE decreased sodium potential (ENa.) and sodium conductance (GNa.). The net Na+ flux decreased due principally to a fall in Na+ flux in the active direction. GE decreased Na(+)-K+ ATPase activity in vitro. Partial replacement of sodium by choline in the outer solution reduced the effect of GE on the skin and substitution of normal Ringer's solution with isethionate Ringer's solution in the outer solution significantly enhanced the effect of GE on the skin. These results indicate that GE decreases active Na transport in the toad skin.
研究了大蒜鳞茎水提取物（GE）对蟾蜍皮肤的作用。当将GE添加到内侧（浆膜侧）溶液中时，可使跨上皮电位差和短路电流分别最大可逆性降低38%和45%。当添加到外侧（黏膜侧）溶液中时，该作用仅部分可逆。艾萨克森的阿米洛利试验表明，GE降低了钠电位（ENa.）和钠电导（GNa.）。净Na+通量下降主要是由于主动方向的Na+通量降低。GE在体外降低了Na(+)-K+ ATP酶活性。外侧溶液中用胆碱部分替代钠可降低GE对皮肤的作用，而外侧溶液中用羟乙基磺酸林格氏液替代正常林格氏液可显著增强GE对皮肤的作用。这些结果表明，GE降低了蟾蜍皮肤中的主动钠转运。
