从大鼠大脑中克隆非多聚腺苷酸化RNA。

Cloning of non-polyadenylated RNAs from rat brain.

作者信息

Harris D A, Sherbany A A

机构信息

Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, NY 10032.

出版信息

Brain Res Mol Brain Res. 1991 Apr;10(1):83-90. doi: 10.1016/0169-328x(91)90059-7.

DOI:10.1016/0169-328x(91)90059-7

PMID:1647486

Abstract

Rodent brain has been reported to contain a fraction of non-polyadenylated (poly(A)-) mRNA that includes about 100,000 different sequences, most of which are not found in the poly(A)+ fraction. We have prepared a cDNA library of low-abundance poly(A)- RNAs from rat brain polysomes, and have characterized three clones in detail. Two of the clones hybridize on Northern blots to poly(A)+ RNAs from brain. Dot blot hybridization and RNase protection assays demonstrate that although the bulk of the RNA complementary to these clones is present in the poly(A)- fraction, a small portion (7-21%) is present in the poly(A)+ fraction. Our results suggest that the poly(A)-mRNA fraction from rat brain may not contain sequences that are different from those in the poly(A)+ fraction.

摘要

据报道，啮齿动物大脑中含有一部分非聚腺苷酸化（poly(A)-）mRNA，其包含约100,000种不同序列，其中大部分在聚腺苷酸化（poly(A)+）部分中未发现。我们从大鼠脑多核糖体中制备了低丰度poly(A)- RNA的cDNA文库，并详细鉴定了三个克隆。其中两个克隆在Northern印迹上与来自大脑的poly(A)+ RNA杂交。斑点印迹杂交和核糖核酸酶保护试验表明，虽然与这些克隆互补的大部分RNA存在于poly(A)-部分中，但一小部分（7-21%）存在于poly(A)+部分中。我们的结果表明，大鼠脑的poly(A)-mRNA部分可能不包含与poly(A)+部分不同的序列。