脂肪酸合成酶cDNA克隆及其mRNA的特性分析

Characterization of fatty acid synthetase cDNA clone and its mRNA.

作者信息

Yan C, Wood E A, Porter J W

出版信息

Biochem Biophys Res Commun. 1985 Feb 15;126(3):1235-41. doi: 10.1016/0006-291x(85)90318-3.

Abstract

Four cDNA clones have been identified by hybrid-select translation to contain the sequences complementary to fatty acid synthetase mRNA. The restriction mapping of these clones indicated that three of these, pFAS-7, pFAS-17 and pFAS-18, have sequences in common, and a fourth, pFAS-15, did not hybridize with the others, suggesting sequence to another region of the mRNA. Northern analysis of cytoplasmic poly(A) +RNA showed the presence of two bands at 9.2 Kb and 8.4 Kb. Similar analysis of nuclear RNA also showed the presence of two bands at 14 and 11 Kb. These probably represent unprocessed transcripts. Southern analysis of genomic DNA digested with EcoRI, BamHI, HindIII and PstI indicate the presence of a single gene copy for fatty acid synthetase.

摘要

通过杂交选择翻译已鉴定出四个cDNA克隆，它们包含与脂肪酸合成酶mRNA互补的序列。这些克隆的限制性图谱表明，其中三个，即pFAS - 7、pFAS - 17和pFAS - 18，具有共同序列，而第四个pFAS - 15与其他克隆不杂交，表明其序列位于mRNA的另一个区域。对细胞质多聚腺苷酸加尾RNA的Northern分析显示在9.2 Kb和8.4 Kb处有两条带。对核RNA的类似分析也显示在14 Kb和11 Kb处有两条带。这些可能代表未加工的转录本。用EcoRI、BamHI、HindIII和PstI消化基因组DNA后的Southern分析表明存在脂肪酸合成酶的单基因拷贝。