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波兰儿童急性淋巴细胞白血病患者免疫球蛋白和T细胞受体（Ig/TCR）基因重排模式——对基于RQ-PCR的微小残留病评估的意义

Pattern of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangements in Polish pediatric acute lymphoblastic leukemia patients--implications for RQ-PCR-based assessment of minimal residual disease.

作者信息

Dawidowska Małgorzata, Derwich Katarzyna, Szczepański Tomasz, Jółkowska Justyna, van der Velden Vincent H J, Wachowiak Jacek, Witt Michał

机构信息

Institute of Human Genetics, Polish Academy of Sciences, Department of Molecular and Clinical Genetics, Strzeszyńska 32, 60 479 Poznań, Poland.

出版信息

Leuk Res. 2006 Sep;30(9):1119-25. doi: 10.1016/j.leukres.2006.01.002. Epub 2006 Feb 14.

DOI:10.1016/j.leukres.2006.01.002

PMID:16476479

Abstract

We studied 23 Polish children with precursor-B-ALL, using PCR-heteroduplex analysis and DNA sequencing, to determine the availability of Ig/TCR gene rearrangements as patient-specific MRD-RQ-PCR targets. We found IGH, IGK-Kde, incomplete TCRD, Vdelta2-Jalpha, TCRG and TCRB rearrangements in 83%, 39%, 61%, 35%, 61% and 13% of patients, respectively. Comparison of Ig/TCR gene rearrangements pattern (frequency and characteristics of rearrangements) in Polish patients with those reported for patients of other European nationalities did not show major differences. These results are the first promising step for further development of MRD study in Polish patients according to current diagnostic standards.

摘要

我们使用聚合酶链反应-异源双链分析和DNA测序技术，对23名波兰前体B细胞急性淋巴细胞白血病患儿进行了研究，以确定Ig/TCR基因重排作为患者特异性微小残留病实时定量聚合酶链反应（MRD-RQ-PCR）靶点的可用性。我们分别在83%、39%、61%、35%、61%和13%的患者中发现了IGH、IGK-Kde、不完全TCRD、Vdelta2-Jalpha、TCRG和TCRB重排。波兰患者的Ig/TCR基因重排模式（重排的频率和特征）与其他欧洲国家患者的报道相比，没有显示出重大差异。这些结果是根据当前诊断标准在波兰患者中进一步开展微小残留病研究的第一个有希望的步骤。