中国成人急性淋巴细胞白血病患者克隆性免疫球蛋白和T细胞受体（Ig/TCR）基因重排模式
The pattern of clonal immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangements in Chinese adult acute lymphoblastic leukemia patients.
作者信息
Yao Li, Chen Zixing, Cen Jiannong, Liang Jianyin, Feng Yufeng, He Jun, Qi Xiaofei, Shen Hongjie
机构信息
Jiangsu Institute of Hematology, The First Affiliated Hospital, Suzhou University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China.
出版信息
Leuk Res. 2008 Nov;32(11):1735-40. doi: 10.1016/j.leukres.2008.03.025. Epub 2008 May 5.
We have studied forty Chinese adult ALL patients at newly diagnosis, using standard primers and protocols of BIOMED-2 multiplex PCR, to determine the feasibility of Ig and TCR gene rearrangements as diagnostic and patient-specific MRD-RQ-PCR targets for molecular monitoring. Clonal IGH, IGK, IGL, TCRB, TCRG and TCRD rearrangements were found in 86%, 22%, 9%, 19%, 77%, 55% of adult patients with B-lineage ALL, respectively. While in T-ALL, clonal IGH, TCRB, TCRG and TCRD rearrangements were detected in 6%, 83%, 78%, 33% of patients. Several specialties in the pattern of Ig/TCR gene rearrangements in Chinese adult ALL patients in comparison with those reported for children and adult patients in other countries have been noted. These results are useful for further MRD-RQ-PCR detection and quantification for all patients.
我们使用BIOMED-2多重PCR的标准引物和方案，对40例新诊断的中国成年急性淋巴细胞白血病（ALL）患者进行了研究，以确定Ig和TCR基因重排作为分子监测的诊断和患者特异性微小残留病定量逆转录聚合酶链反应（MRD-RQ-PCR）靶点的可行性。在B系ALL成年患者中，分别有86%、22%、9%、19%、77%、55%检测到克隆性IGH、IGK、IGL、TCRB、TCRG和TCRD重排。而在T-ALL中，6%、83%、78%、33%的患者检测到克隆性IGH、TCRB、TCRG和TCRD重排。与其他国家报道的儿童和成年患者相比，中国成年ALL患者的Ig/TCR基因重排模式存在一些特点。这些结果有助于对所有患者进一步进行MRD-RQ-PCR检测和定量分析。
Zotero 插件