Pin1与Nek6的相互作用及其在中国肝细胞癌患者中的表达相关性研究
Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients.
作者信息
Chen Jian, Li Li, Zhang Yuanyuan, Yang Huirong, Wei Youheng, Zhang Lin, Liu Xianghua, Yu Long
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
出版信息
Biochem Biophys Res Commun. 2006 Mar 24;341(4):1059-65. doi: 10.1016/j.bbrc.2005.12.228. Epub 2006 Jan 25.
The peptidyl-prolyl isomerase Pin1 is prevalently overexpressed in human cancers and is regarded as a new diagnostic and therapeutic target. Pin1 interacts with several proteins involved in cell cycle events in a phosphorylation-dependent manner. Among them, NIMA (never in mitosis, gene A) was first identified to interact with Pin1. In this report, we found that Pin1 could interact with Nek6, one of the human NIMA-related kinases (Neks). This interaction was confirmed by GST pull-down assay, which was further confirmed by immunoprecipitation experiments, as well as immunofluorescence colocalization. We further studied Pin1 and Nek6 mRNA level in 40 pairs of hepatocellular carcinoma cases, finding significant correlations between Nek6 and Pin1 mRNA expression levels in these samples.
肽基脯氨酰顺反异构酶Pin1在人类癌症中普遍过度表达，被视为一种新的诊断和治疗靶点。Pin1以磷酸化依赖的方式与几种参与细胞周期事件的蛋白质相互作用。其中，NIMA（从不进行有丝分裂，基因A）最早被鉴定为与Pin1相互作用。在本报告中，我们发现Pin1可以与人类NIMA相关激酶（Neks）之一的Nek6相互作用。这种相互作用通过谷胱甘肽S-转移酶（GST）下拉试验得到证实，免疫沉淀实验以及免疫荧光共定位进一步证实了这一点。我们进一步研究了40对肝细胞癌病例中Pin1和Nek6的mRNA水平，发现这些样本中Nek6和Pin1的mRNA表达水平之间存在显著相关性。
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