Sheng Rui-Yan, Li Peng-Min, Xue Guo-Xi, Zhao Xin-Xi, Gao Hui-Yuan
Department of Plant Science, Shandong Agricultural University, Tai'an 271018, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Feb;32(1):87-93.
Choline chloride 1.07 mmol/L treatment diminished the saturated lipid contents of the fatty acid components mainly the phosphatidylglycerol (PG) resulting in the decrease of the saturation of lipid (Table 1), declined the permeability of cell membrane and the production of MDA from lipid peroxidation (Fig.2) in the cucumber seedling leaves under low temperature and weak light (6 degrees C, PFD 100 micromol m(-2) s(-1)). Furthermore, the choline chloride treatment alleviated the degradation of chlorophyll pigments especially chlorophyll b, the decrease in maximum photochemical efficiency (Fv/Fm), the capture efficiency of excited energy (Fv'/Fm'), the photochemical quenching coefficient (q(p)) and the actual photochemical efficiency (Phi PSII) of PSII (Table 2, Fig.3A, B & C), and decreases in activity of antioxidant enzymes such as POD, APX and CAT (Fig.4) in chilled leaves under weak light. In addition, choline chloride treatment increased the non-photochemical quenching (NPQ) (Fig.3D) and the proline content (Fig.5) in chilled leaves under weak light. The above results indicate that choline chloride protected the cell membrane and the photosynthetic apparatus in cucumber seedling leaves from chilling stress in weak light.
1.07毫摩尔/升的氯化胆碱处理降低了脂肪酸成分中的饱和脂质含量，主要是磷脂酰甘油（PG），导致脂质饱和度下降（表1），在低温弱光（6摄氏度，光合光子通量密度100微摩尔·米-2·秒-1）条件下，黄瓜幼苗叶片的细胞膜通透性以及脂质过氧化产生的丙二醛含量降低（图2）。此外，氯化胆碱处理减轻了叶绿素尤其是叶绿素b的降解、最大光化学效率（Fv/Fm）的降低、激发能捕获效率（Fv'/Fm'）、光化学猝灭系数（q(p)）以及光系统II的实际光化学效率（Phi PSII）的下降（表2，图3A、B和C），并减少了弱光下低温叶片中过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）等抗氧化酶的活性（图4）。此外，氯化胆碱处理增加了弱光下低温叶片的非光化学猝灭（NPQ）（图3D）和脯氨酸含量（图5）。上述结果表明，氯化胆碱保护黄瓜幼苗叶片的细胞膜和光合机构免受弱光下低温胁迫的影响。
